Rasagiline, a monoamine oxidase-B inhibitor, protects NGF-differentiated
PC12 cells against oxygen-glucose deprivation.

In our in vitro model, rasagiline a selective irreversible monoamine
oxidase-B (MAO-B) inhibitor, protected nerve growth factor
(NGF)-differentiated PC12 cells from cell death under oxygen and glucose
deprivation (OGD).

The severity of the OGD insult, as expressed by cell death, was
time-dependent.

Exposure of the cells to OGD for 3 hr followed by 18 hr of reoxygenation
caused about 30-40% cell death.

Under these conditions, the neuroprotective effect of rasagiline was
dose-dependent: rasagiline reducing OGD-induced cell death by 68% and 80%
at 100 nM and 1 microM, respectively.

The neuroprotective effect of rasagiline was also observed when added after
the OGD insult (55% reduction in cell death).

Under rasagiline treatment, there was a lesser decrease in ATP content in
cultures exposed to OGD compared with that in untreated cultures.

OGD followed by reoxygenation resulted in a several fold increase in PGE(2)
release into the extracellular medium.

Rasagiline (100 nM-1 microM) markedly inhibited OGD-induced PGE(2) release.

Clorgyline, a monoamine oxidase-A (MAO-A) inhibitor, did not protect
NGF-differentiated PC12 cells against OGD-induced cell death.

As NGF-differentiated PC12 cells contain exclusively MAO type A, these data
suggest that the neuroprotective effect of rasagiline under OGD conditions
is independent of MAO inhibition.

J Neurosci Res 1999 Nov 1;58(3):456-63
Abu-Raya S, Blaugrund E, Trembovler V, Shilderman-Bloch E, Shohami E,
Lazarovici P
School of Pharmacy, Faculty of Medicine, The Hebrew University, Jerusalem,
Israel
PMID: 10518120

http://www.ncbi.nlm.nih.gov/PubMed/

janet paterson, an akinetic rigid subtype parkie
53 now / 44 dx cd / 43 onset cd / 41 dx pd / 37 onset pd
TEL: 613 256 8340 SMAIL: PO Box 171 Almonte Ontario K0A 1A0 Canada
EMAIL: [log in to unmask] URL: http://www.geocities.com/janet313/