From The Times
August 30, 2008

Meet the man with 250 brains
David Dexter uses donated brains to unlock the secrets of Parkinson's 
disease

John Naish
David Dexter pulls from under his lab desk a small lidded bucket, the type 
that DIY stores use to sell bumper packs of filler. Inside, floating in 
clear fluid, is a human brain: grey, cold, murky. "This is quite a 
lightweight one," he says, fishing it into his purple-gloved grasp. "When 
they first come in they are fresh and pink. This has been soaking in 
fixative for four weeks." For squeamish laypersons like me, it feels a 
vaguely macabre way to tackle one of the most common diseases of ageing.
Dexter, a quiet, shaven-headed scientist, is the man with 251 brains. And he 
would love it if you gave him yours - once you've finished using it. He is 
the scientific director of the Parkinson's Disease Society Tissue Bank, at 
Imperial College London. The bank is at the leading edge of British efforts 
to discover the causes and cures for this debilitating neurological 
disorder.
But even highly intelligent clinicians such as Dexter can lack vital grey 
matter. The bank collects and dissects for study the brains of people who 
have died with Parkinson's. But it needs just as many "control" brains - 
those that belong to people who did not apparently have the disease - so 
that scientists have a direct counterpart of the Parkinson's brain they are 
studying, in terms of age, gender and time elapsed between death and 
preservation.
The bank is funded by the Parkinson's Disease Society at a cost of £25,000 a 
year. Since it opened five and a half years ago, it has collected 250 
donated brains, only 17 of which are controls. "We are collecting four or 
five brains a month. We tend to get a peak on cold winter days and summer 
heat waves," Dexter says. "The lack of control brains is our biggest problem 
because every time we send out tissues, we need to send out control tissues 
as well. His colleague Kirsten Goldring, who manages the donor programme, 
adds: "It's odd, but when people sign on with us as controls, they seem to 
live for ever."
Related Links
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A race against time
The fact that brain tissue certainly does not last for ever is one of the 
greatest practical challenges facing the bank. As an old medical saying has 
it: "After death, of all the soft tissues, the brain's the first to vanish, 
the uterus last." Dexter explains how news of a donor's demise sparks a race 
against time, geography and bureaucracy. "As soon as someone dies, his or 
her brain cells start dying. We need to get the preservation procedure done 
quickly. We need to get the brain into the lab within 24 hours. Our 
co-ordinator is on call 24 hours every day. We've managed to get brains to 
London on time from places such as Northern Ireland and Jersey." For that to 
happen, all the paperwork must be in order. "It can be infuriating if we 
don't make it because, for example, a locum doctor only 50 miles down the 
road won't sign the death certificate and wants to wait until the patient's 
usual GP is found," says Dexter. The process is expedited if the donor's kin 
know of his or her wishes. The programme has its own donor cards; standard 
organ-donor cards do not cover brains as they are not going to be used 
clinically in transplants.
Anna Lowe, 31, a management consultant living in Balham, South London, is 
one of the latest non-Parkinson's patients to decide to donate her brain. 
"My grandfather died three weeks ago at the age of 78," she says. "I had 
never thought about donating, but just before he died I learnt of his plans. 
He had agreed to donate his brain about three years ago. The awareness of 
the brain bank motivated me to do it, too. He had told his wife about it, 
and he had all the right documents lodged. My grandfather was a retired 
psychiatrist and had treated people with Parkinson's. When he was diagnosed 
with it, he was very interested to keep up with all the latest research, and 
that's how he learnt about the work of the tissue bank."
Lowe, whose grandfather's condition was diagnosed about seven years ago, 
believes that this decision to donate has proved a comfort to her 
grandmother. "It was something that he very much wanted," she says. "He was 
quite prag-matic about the physical body, being a doctor, and he valued the 
research that his brain would help the scientists to do. It was a comfort to 
all of us that him donating his brain could help others." As for her 
decision to donate, "I didn't have to think too much about it," she says. 
"If I'm dead, I won't know about it. And I have already signed up as an 
organ donor for other bits and pieces. I'm quite young and it seems a long 
way off. It feels like something useful I could do. Dying and being a donor 
to help others is a wonderful thing. The most important thing is to tell 
your kin so they are aware of your wishes if they are asked for their 
consent."
Dissecting brains is a three-person job
Over lunchtime sandwiches, Dexter explains how dissecting a donated brain 
quickly and efficiently is a three-person job. The most important section is 
the substantia nigra, a thumbnail-sized region located at the base of the 
brain. On a glass laboratory slide it resembles a flattened-out moth. Here 
the vital chemical, dopamine, is synthesised. It is responsible for creating 
control of our finer physical movements. Dexter shows me how, on a slide of 
a section of a healthy substantia nigra, there is a clear black line where 
the dopamine-producing cells are sited. On a slide from a Parkinson's 
sufferer's brain, that black line is a light grey smudge. "This is the main 
area affected by Parkinson's," says Dexter, "So you've got lots of 
researchers wanting to look at the same small thing."
By comparing Parkinson's brains with normal brains, scientists can tell 
which cell-death processes are caused by disease, and which are part of 
ageing. This helps scientists to understand the condition better, which 
enables them to develop more effective drugs and gene therapies. But brains 
are mysterious things. They come in very different sizes and shapes. The 
smallest donated adult brain was 700g.
"The largest was more than twice that size," smiles Dexter. "We do play a 
guessing game about the gender, but generally female brains are smaller." 
Goldring feels compelled to interject: "They are more compact and better 
organised." Regardless of their gender, brains remain something of a mystery 
to medics while we are still alive. Even a diagnosis of Parkinson's disease 
is often wrong, as Dexter's team frequently discovers. "We do a detailed 
neurophysiological assessment of the brain to see whether they really did 
have Parkinson's. Only about 70 per cent of the people diagnosed as dying 
from the disease actually had it," he says. "Others have conditions such as 
progressive supernuclear palsy. Some have Alzheimer's as well as 
Parkinson's: it can be a multitude of syndromes."
Even apparently normal brains can harbour surprises, Dexter adds. "Often the 
control brains can give us an insight into the pre-symptomatic stages of 
Parkinson's. About 7 per cent of people in the general population have 
Parkinson's at the pre-clinical stage. But their owners frequently don't 
know get to know this, as many of them die of something else before they 
ever go on to develop Parkinsonian symptoms."
"We just cut a flap at the back of the head"
I ask Dexter something that fascinates me - whether any people fear that by 
donating their brains, they might lose a part of their souls. He doesn't see 
this as an issue - and it's not something that will prevent him from 
donating his brain. But vanity can prove a potential obstacle, he says: 
"Some possible donors are worried that their bodies will be disfigured and 
they won't be able to be displayed in an open casket. But looking at the 
dead donor's face, you would never know. We just cut a flap at the back of 
the head, peel the skin over the top, cut a hole in the top of the skull, 
take the brain out, and then put everything back." He mimes the procedure, 
then glances at my whitening face and smiles: "Oh," he says, "You're still 
having your lunch."
For more information on becoming a donor, contact the UK Parkinson's Disease 
Society Tissue Bank: 0207-594 9732, [log in to unmask] 
www.parkinsons.org.uk
Brains in numbers
120,000 people with Parkinson's in the UK
30,000 members of the Parkinson's Disease Society
600 number of people currently signed up to donate their brains
-80C optimum temperature for preserving a donated brain

Rayilyn Brown
Director AZNPF
Arizona Chapter National Parkinson Foundation
[log in to unmask] 

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