Eleanor Noone wrote:
> In a message dated 1/20/00 2:44:11 AM EST, [log in to unmask] writes:
>
> << it is a gift and you should accept it graciously. Using the
> stem cells is NOT killing, it is preserving and enhancing life, as long as
> abortions are not being performed solely to obtain the cells. I can't believe
> that God would want His children to suffer, when it is preventable. >>
>
> I've been trying to figure out
> how to say just what Ken has, but I could never have said it so eloquently.
the following company is poised for production
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Biotechnol Bioeng 1999 Dec 5;65(5):589-99
Extended serial passaging of mammalian neural stem cells in suspension
bioreactors.
Kallos MS, Behie LA, Vescovi AL
Pharmaceutical Production Research Facility (PPRF), Faculty of Engineering,
University of Calgary, 2500 University Drive NW, Calgary, Alberta T2N 1N4,
Canada.
Neural stem cells (NSCs) are primitive cells that are the "parent" cells of
all the cells in the central nervous system (CNS). Their discovery in 1992
opened the door to a multitude of potential therapies and treatments to cure
neurodegenerative diseases such as Parkinson's disease, multiple sclerosis,
and Huntington's disease, which affect millions of people worldwide and cost
billions of dollars in health care each year. This study proposes optimal
serial passaging protocols so that mammalian neural stem cells can
effectively be grown in suspension culture. We examined stationary culture
passaging protocols and developed our own optimal procedure. Also examined
was the effect of serially cultivating the neural stem cells in suspension
culture for an extended period of time. The cells were grown for over 35
days in suspension with an overall multiplication ratio of over 10(7) with
no decrease in growth rate, maximum cell density, or viability. The cells
also remained karyotypically normal through 25 doublings and retained their
ability to be differentiated into all the major cell types of the
CNS-neurons, astrocytes, and oligodendrocytes. For the first time, mammalian
neural stem cells were grown on a larger scale in suspension culture and
maintained their stem cell characteristics. A semicontinuous scheme for
large-scale production is also presented. Copyright 1999 John Wiley & Sons,
Inc.
PMID: 10516585, UI: 99447457
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Ray Strand
mailto:[log in to unmask]
48/47/45?
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...on the edge of the prairie abyss ......................
