List:
A one percent yield may seem small, but, there are methods for purification
and further manipulation in culture.
This is what a company would like to cash in on...and eventually will.
"Human mesencephalic neural precursor cells" --
sometimes referred to as adult stem cells.
But, embryonic cells could also be used...they are the precursers to everything.
Undestanding of the effect and timing and withdrawal of various
growth factors in vitro and in vivo is necessary for
successful transplantation control.
Ray
.......................................................
Exp Neurol 2001 Aug;170(2):317-25
Long-term proliferation and dopaminergic differentiation of human mesencephalic
neural precursor cells.
Storch A, Paul G, Csete M, Boehm BO, Carvey PM, Kupsch A, Schwarz J.
Department of Neurology, University of Ulm Medical School, Ulm, Germany
We report on generation of dopamine neurons from long-term cultures of human
fetal mesencephalic precursor cells. These CNS precursor cells were successfully
expanded in vitro using the mitogens epidermal growth factor (EGF) and
fibroblast growth factor-2 (FGF-2). Incubation of these cultures in 3%
atmospheric oxygen resulted in higher cellular yields than room air. Following
incubation in differentiation media containing interleukin (IL)-1b (IL-1b),
IL-11, leukemia inhibitory factor (LIF), and glial cell line-derived
neurotrophic factor (GDNF), up to 1% of the precursor cells converted into cells
immunoreactive for tyrosine hydroxylase (TH), a marker for dopamine
neurons. The
TH immunoreactive cells exhibited morphological and functional properties
characteristic of dopamine neurons in culture. These precursor cells might serve
as a useful source of human dopamine neurons for studying the development and
degeneration of human dopamine neurons and may further serve as a continuous,
on-demand source of cells for therapeutic transplantation in patients with
Parkinson's disease. Copyright 2001 Academic Press.
PMID: 11476598 [PubMed - in process
.......................................................
Ray Strand
Prairie Sky Design
-----------------( on the Edge of the Prairie Abyss )---------------
when the sky is clear
the ground is visible
49/dx PD 2 yrs/40? onset
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