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Hello List members, I have been silent for a long time but I couldn’t resist doing a little follow up on the Nitric oxide discovery at John’s Hopkins. After reading about the damage caused by too much nitric oxide in PWP’s I did a little search and discovered that Interferon-beta-1-b (IFN-ß) decreases induced Nitric Oxide (NO) production by a human astrocytoma cell line. Is this interferon available to PWP’s and can it be used clinically today? Wouldn’t that be a revolutionary change in PD treatment. Regards Bob Martone ------------------------------------------ Interferon-beta-1-b (IFN-ß) Decreases Induced Nitric Oxide (NO) Production By A Human Astrocytoma Cell Line Guthikonda P, Baker J, Mattson DH J NeuroImmunol 1998 Mar 1;82(2):133-9 Univ of Pennsylvania, Student School of Dentistry, Philadelphia 19104, USA _____ <http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Display&d opt=pubmed_pubmed&from_uid=98246901> UI# 98246901 Abstract Inducible Nitric Oxide Synthase (iNOS) is expressed by <http://www.albany.net/~tjc/gloss1-g.html#Astrocyte> Astrocytes in DeMyelinating regions of Multiple Sclerosis ( <http://www.albany.net/~tjc/gloss1-m.html#MS> MS) Brain plaques, suggesting that NO contributes to MS pathology. Since the ImmunoSuppressive <http://www.albany.net/~tjc/gloss1-c.html#Cytokines> Cytokine IFN-ß ameliorates MS disease activity, it is of interest to assess the modulatory role of IFN-ß on NO production. We studied the effects of IFN-ß, as well as Dexamethasone, IL-10, and Transforming Growth Factor-ß (TGF-ß), on Cytokine-induced NO production by the human Astrocytoma cell line, A172. L-NMMA and Aminoguanidine, competitive inhibitors of iNOS suppressed <http://www.albany.net/~tjc/nitric-oxide_axons.html#1#1> NO production as measured by the NO byproduct, Nitrite, as did <http://www.albany.net/~tjc/gloss1-i.html#beta> Interferon-ß. Dexamethasone enhanced NO production, and IFN-ß decreased the amount of the enhancement. Neither <http://www.albany.net/~tjc/interleukin.html#IL-10> IL-10 nor TGF-ß inhibited Nitrite production. The therapeutic effect of IFN-ß in MS may be partly due to suppression of Pathogenic NO production. ---------------------------------------------------------------------- To sign-off Parkinsn send a message to: mailto:[log in to unmask] In the body of the message put: signoff parkinsn