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WAY, WAY, WAY too many big words, especially for those of us with a primate brain!!!! I'll bet even our Dr.'s on the list had trouble with this one. LMHO Is there anyone out there who can give us a translation? How's this for a starter? "These scientists conducted this study where they put some modified rat stuff into a monkey's brain - - - - " > ---------- > From: janet paterson[SMTP:[log in to unmask]] > Sent: Friday, September 10, 1999 9:58 AM > Subject: PMID: 10478724: Reaction of primate brain to grafted PC12 > cells. > > Evaluation of reaction of primate brain to grafted PC12 cells. > > Intrastriatal implantation of polymer-encapsulated PC12 cells, which > constitute a dopaminergic cell line derived from rat pheochromocytoma, has > proved useful for ameliorating parkinsonian symptoms in several kinds of > animals. > > In considering the clinical application of this technique, we should make > sure that PC12 cells are rejected completely by the host immune system in > case the capsule breaks. > > In the present study, unencapsulated PC12 cells were injected into the > brain of Japanese monkeys (Macaca fusscata). > > Histological [hematoxylin-eosin (H&E), Nissl] and immunocytochemical > [tyrosine hydroxylase (TH), and glial fibrillary acidic protein (GFAP)] > analyses were performed 1, 2, 4, and 8 weeks after transplantation. > > Also, encapsulated PC12 cells were transplanted into the brain of another > group of Japanese monkeys to investigate the host reaction to the capsule > and to confirm that the encapsulated PC12 cells continue to survive in the > host brain. > > H&E and GFAP staining were performed 2, 4, and 8 weeks after > transplantation. > > L-DOPA and dopamine release from the explanted capsules was measured by > high performance liquid chromatography. > > Magnetic resonance imaging was performed in both unencapsulated and > encapsulated PC12 cell grafted groups. > > Although the xenografted unencapsulated cells formed a small cluster at 1 > and 2 weeks after implantation, very few and no viable PC12 cells remained > at 4 and 8 weeks, respectively. > > The reaction of the host towards the xenograft gradually decreased. > > Encapsulated PC12 cells retrieved from the host brain were found to > release > L-DOPA and dopamine continuously even 8 weeks after implantation. > > The host reaction to the PC12-loaded capsule was much weaker than that to > the unencapsulated PC12 cells, and decreased with time. > > These results indicate that encapsulated PC12 cell transplantation is an > effective and safe strategy for the treatment of Parkinson's disease. > > > Cell Transplant 1999 Jul-Aug;8(4):427-30 > Yoshida H, Date I, Shingo T, Fujiwara K, Miyoshi Y, Furuta T, Ohmoto T > Okayama University Medical School, Okayama-ken, Japan. > <[log in to unmask]> > PMID: 10478724, UI: 99405829 > > <http://www.ncbi.nlm.nih.gov/PubMed/> > > janet paterson > 52 now / 41 dx / 37 onset > 613 256 8340 po box 171 almonte ontario canada K0A 1A0 > a new voice: <http://www.geocities.com/SoHo/Village/6263/> > <[log in to unmask]> >