-----Oorspronkelijk bericht-----
Van: janet paterson <[log in to unmask]>
Aan: [log in to unmask] <[log in to unmask]>
Datum: vrijdag 10 september 1999 19:24
Onderwerp: PMID: 10478724: Reaction of primate brain to grafted PC12 cells.
"Simple" translation for Darwin and other primates.
Hans. 8<{))))))))}}
How does a primate brain react to the implantation of PC12 cells?
Researchers implantated a capsule containing PC12 cells from rats.
These PC12 cells produce L-dopa and dopamine.
The PC12 cells cannot leave the capsule, while the L-dopa and dopamine can.
It was shown that in this way PD symptoms in animals improved.
In the present study PC12 cells without a capsule were injected into the
brain of one group of Japanese monkeys.
Laboratory analyses were performed 1, 2, 4, and 8 weeks after implantation.
Also PC12 cells within a capsule were implanted into the brain of another
group of Japanese monkeys to investigate the host reaction to the capsule
and to confirm that the encapsulated PC12 cells continue to survive in the
host brain.
Laboratory analyses were performed 2, 4, and 8 weeks after implantation.
L-DOPA and dopamine release from the implanted capsules was measured by a
special methode of measuring their amounts.
Magnetic resonance imaging was performed in both unencapsulated and
encapsulated PC12 cell groups.
From the unencapsulated cells very few and no viable PC12 cells remained
after a few weeks.
Encapsulated PC12 cells were found to release L-DOPA and dopamine
continuously even 8 weeks after implantation.
The host reaction to the PC12-loaded capsule was much weaker than that to
the unencapsulated PC12 cells, and decreased with time.
These results indicate that encapsulated PC12 cell implantation is an
effective and safe strategy for the treatment of Parkinson's disease.
